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wild type cowpox virus strain brighton  (ATCC)


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    ATCC wild type cowpox virus strain brighton
    Yeast cells were co-transformed with fragmented pYCC3-VACV plasmid DNA together with <t>CPXV</t> and RPXV genomic DNA. Each resulting yeast colony is expected to contain a distinct chimeric poxvirus sequence (pYCC3-cPOX) generated through homologous recombination. DNA extracted from individual colonies was subsequently transfected into human cells pre-infected with the helper virus MVA, enabling the production of chimeric infectious viral particles.
    Wild Type Cowpox Virus Strain Brighton, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 70 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wild+type+cowpox+virus+strain+brighton/bio_rxiv__64898__2026__03__06__710085-193-13-19?v=ATCC
    Average 94 stars, based on 70 article reviews
    wild type cowpox virus strain brighton - by Bioz Stars, 2026-07
    94/100 stars

    Images

    1) Product Images from "Synthetic Genome Shuffling of Poxviruses through Yeast for Next-Generation Oncolytic Platforms"

    Article Title: Synthetic Genome Shuffling of Poxviruses through Yeast for Next-Generation Oncolytic Platforms

    Journal: bioRxiv

    doi: 10.64898/2026.03.06.710085

    Yeast cells were co-transformed with fragmented pYCC3-VACV plasmid DNA together with CPXV and RPXV genomic DNA. Each resulting yeast colony is expected to contain a distinct chimeric poxvirus sequence (pYCC3-cPOX) generated through homologous recombination. DNA extracted from individual colonies was subsequently transfected into human cells pre-infected with the helper virus MVA, enabling the production of chimeric infectious viral particles.
    Figure Legend Snippet: Yeast cells were co-transformed with fragmented pYCC3-VACV plasmid DNA together with CPXV and RPXV genomic DNA. Each resulting yeast colony is expected to contain a distinct chimeric poxvirus sequence (pYCC3-cPOX) generated through homologous recombination. DNA extracted from individual colonies was subsequently transfected into human cells pre-infected with the helper virus MVA, enabling the production of chimeric infectious viral particles.

    Techniques Used: Transformation Assay, Plasmid Preparation, Sequencing, Generated, Homologous Recombination, Transfection, Infection, Virus

    (A) Brightfield microscopy images of plaque assays performed on HeLa cells infected with parental viruses (VACV, CPXV, RPXV) and with chimeric viruses rescued from TAR-shuffling clones (cPOX01-02, cPOX02-03, cPOX04-12, cPOX05-14, and cPOX06-18). HeLa cells were seeded at 5 × 10⁵ cells per well in 6-well plates and infected at a multiplicity of infection (MOI) of 10⁻⁵. Images were acquired 48 h post-infection at 4× magnification. (B) Representative comet-shaped plaques observed in A549 cells. A549 monolayers were infected with the indicated viruses, incubated for 48 h, and stained with crystal violet to visualize comet formation, reflecting enhanced viral spread
    Figure Legend Snippet: (A) Brightfield microscopy images of plaque assays performed on HeLa cells infected with parental viruses (VACV, CPXV, RPXV) and with chimeric viruses rescued from TAR-shuffling clones (cPOX01-02, cPOX02-03, cPOX04-12, cPOX05-14, and cPOX06-18). HeLa cells were seeded at 5 × 10⁵ cells per well in 6-well plates and infected at a multiplicity of infection (MOI) of 10⁻⁵. Images were acquired 48 h post-infection at 4× magnification. (B) Representative comet-shaped plaques observed in A549 cells. A549 monolayers were infected with the indicated viruses, incubated for 48 h, and stained with crystal violet to visualize comet formation, reflecting enhanced viral spread

    Techniques Used: Microscopy, Infection, Clone Assay, Incubation, Staining



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    wild type cowpox virus strain brighton  (ATCC)
    94
    ATCC wild type cowpox virus strain brighton
    Yeast cells were co-transformed with fragmented pYCC3-VACV plasmid DNA together with <t>CPXV</t> and RPXV genomic DNA. Each resulting yeast colony is expected to contain a distinct chimeric poxvirus sequence (pYCC3-cPOX) generated through homologous recombination. DNA extracted from individual colonies was subsequently transfected into human cells pre-infected with the helper virus MVA, enabling the production of chimeric infectious viral particles.
    Wild Type Cowpox Virus Strain Brighton, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wild+type+cowpox+virus+strain+brighton/bio_rxiv__64898__2026__03__06__710085-193-13-19?v=ATCC
    Average 94 stars, based on 1 article reviews
    wild type cowpox virus strain brighton - by Bioz Stars, 2026-07
    94/100 stars
    		  Buy from Supplier

    Image Search Results


    Yeast cells were co-transformed with fragmented pYCC3-VACV plasmid DNA together with CPXV and RPXV genomic DNA. Each resulting yeast colony is expected to contain a distinct chimeric poxvirus sequence (pYCC3-cPOX) generated through homologous recombination. DNA extracted from individual colonies was subsequently transfected into human cells pre-infected with the helper virus MVA, enabling the production of chimeric infectious viral particles.

    Journal: bioRxiv

    Article Title: Synthetic Genome Shuffling of Poxviruses through Yeast for Next-Generation Oncolytic Platforms

    doi: 10.64898/2026.03.06.710085

    Figure Lengend Snippet: Yeast cells were co-transformed with fragmented pYCC3-VACV plasmid DNA together with CPXV and RPXV genomic DNA. Each resulting yeast colony is expected to contain a distinct chimeric poxvirus sequence (pYCC3-cPOX) generated through homologous recombination. DNA extracted from individual colonies was subsequently transfected into human cells pre-infected with the helper virus MVA, enabling the production of chimeric infectious viral particles.

    Article Snippet: Wild-type Vaccinia virus strain Copenhagen (VACV) was obtained from Institut Mérieux (France), while wild-type Cowpox virus strain Brighton (CPXV; ATCC VR-302) and wild-type Rabbitpox virus strain Utrecht (RPXV; ATCC VR-1591) were obtained from ATCC (USA).

    Techniques: Transformation Assay, Plasmid Preparation, Sequencing, Generated, Homologous Recombination, Transfection, Infection, Virus

    (A) Brightfield microscopy images of plaque assays performed on HeLa cells infected with parental viruses (VACV, CPXV, RPXV) and with chimeric viruses rescued from TAR-shuffling clones (cPOX01-02, cPOX02-03, cPOX04-12, cPOX05-14, and cPOX06-18). HeLa cells were seeded at 5 × 10⁵ cells per well in 6-well plates and infected at a multiplicity of infection (MOI) of 10⁻⁵. Images were acquired 48 h post-infection at 4× magnification. (B) Representative comet-shaped plaques observed in A549 cells. A549 monolayers were infected with the indicated viruses, incubated for 48 h, and stained with crystal violet to visualize comet formation, reflecting enhanced viral spread

    Journal: bioRxiv

    Article Title: Synthetic Genome Shuffling of Poxviruses through Yeast for Next-Generation Oncolytic Platforms

    doi: 10.64898/2026.03.06.710085

    Figure Lengend Snippet: (A) Brightfield microscopy images of plaque assays performed on HeLa cells infected with parental viruses (VACV, CPXV, RPXV) and with chimeric viruses rescued from TAR-shuffling clones (cPOX01-02, cPOX02-03, cPOX04-12, cPOX05-14, and cPOX06-18). HeLa cells were seeded at 5 × 10⁵ cells per well in 6-well plates and infected at a multiplicity of infection (MOI) of 10⁻⁵. Images were acquired 48 h post-infection at 4× magnification. (B) Representative comet-shaped plaques observed in A549 cells. A549 monolayers were infected with the indicated viruses, incubated for 48 h, and stained with crystal violet to visualize comet formation, reflecting enhanced viral spread

    Article Snippet: Wild-type Vaccinia virus strain Copenhagen (VACV) was obtained from Institut Mérieux (France), while wild-type Cowpox virus strain Brighton (CPXV; ATCC VR-302) and wild-type Rabbitpox virus strain Utrecht (RPXV; ATCC VR-1591) were obtained from ATCC (USA).

    Techniques: Microscopy, Infection, Clone Assay, Incubation, Staining